sigh. i'm so sorry to ck. T_T i really missed out that word "microinjection of EGTA". didn't mean to let you feel that you were just wasting your time discussing the tutorial with me. i'm just so blurred today. dont be angry. T_T but it's still relevant to the questions. just that i missed out that EGTA is in the cell. sigh. cannot sleep already. been self-reprimanding since you typed out that "ARRGGGGGG!!!" T_T sorry! hontou ni gomenasai.